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Expression Vector Cell Pool Sequencing Report Stable Knockdown Cell Line

CELLFREE SCIENCE CO., LTD.

Expression Vector Cell Pool Sequencing Report Stable Knockdown Cell Line

Brand Name : CELLFREE

Model Number : CF-PR-011

Delivery Time : 2~3 months

Packaging Details : vial

Application : Research, Industrial Production

Deliverables for researchent : Single cell clone, cell pool

Timeline : 3 months

Deliverables for Industry : Expression vector

Report : Sequencing report

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Introduction

 

Stable cell lines are used in a variety of research applications such as recombinant protein and antibody production, assay development, gene editing, functional studies and more. CELLFREE offer comprehensive stable cell line development capabilities that either begin with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging CELLFREE's top quality stable cell line services.

 

Cell Line Construction for Research

 

We can insert the gene you are interested in into the genome of any cell and screen the stably integrated monoclonal cell line which can be applied in many research fields, such as signal transduction, drug target study, immunotherapy drug development, etc. We have successfully constructed cell lines in dozens of kinds of cells. All you need to do is to provide the gene sequence, and we will complete the screening of cell lines as required.

 

              Service content

 

Content

Deliverables

Timeline

Cell Line Construction Single cell clone 3 months
Cell pool 2 months
 

              Service Process

 

  • Gene synthesis
  • Plasmid Max-Prep
  • Deliver the cell pool
  • Antibiotics screening
  • Amplification
  • Delivered Single clone

 

Cell Line Development for Industrial Production

 

The cell for stable cell line development is CHO cell. The CHO cells that are directionally domesticated for our self-developed highly-expressed proEM system show high productivity and cell density in the production of recombinant protein. It can also be domesticated on the basis of client’s special culture medium components. It has excellent growth and metabolism characteristics. Whether in shake flask, wave or bioreactor, its strong growth curve lays a solid foundation for high yield of protein.

 

              Service content

 

Process

Deliverables

Timeline

Gene Synthesis

Codon optimization

Gene synthesis

Vector construction

Plasmid Max-Prep

Expression vector

Sequencing report

 

 

 

 

1 Cell Line

tubes/plant, 1×107cells/ml)

4 weeks
Cell Line Development

Cell transfection

Expression evaluation

MTX/MSX amplification

Single cell clone screening

3 months



Product Tags:

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Sequencing Report Stable Knockdown Cell Line

      

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