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200 µL Lentivirus Stock Custom Lentivirus Production Molecular Genetic Analysis

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Cell Free Protein [10]
Biobasic Gene Synthesis [10]
Oligo Synthesis [10]
Nucleolus Rna Synthesis [10]
Plasmid DNA Preparation [6]
Antibody Discovery [12]
Cellular Molecular Biology [10]
Crispr Cas Gene Editing [12]
Protein Expression And Purification [12]
Molecular Genetic Analysis [10]

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200 µL Lentivirus Stock Custom Lentivirus Production Molecular Genetic Analysis

Brand Name : CELLFREE

Model Number : CF-GA-009

Delivery Time : 3 week

Packaging Details : vial

Starting information : Target gene information

Report : Experiment report

Small-scale lentivirus packaging : 100 µL

Large-scale lentivirus packaging : 200 µL

Deliverable : Lentivirus stock

Lentivirus purification : Ultracentrifugation.chromatography

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Introduction

With the rapid development of the theory and technology of molecular biology (especially the recombinant DNA technology), people can construct various vectors, clone and analyze target genes in the laboratory, so that diseases can be deeply studied at the molecular level. Therefore, gene diagnosis, gene therapy and other technologies are born, so it is particularly critical to select the appropriate and efficient gene delivery medium system, and viral vector has become the hot spot of gene therapy vector research at present. We can provide adenovirus, lentivirus packaging technical services.

CELLFREE offers a lentiviral packaging service for the production of high-quality, high-titer, ready-to-use lentiviral particles. Using a lentivector construct, this service produces VSV-G pseudotyped lentiviral particles at the specified titer level measured as active infectious units (IFU/ml) in just three to four weeks.

You can order lentiviral packaging services using lentivectors provided by you as well as lentivectors constructed by us to obtain ready-to-use particles at various titer levels. We are also happy to customize your order, but please be aware that the prices and delivery times will vary based on the complexity of the order.

Benefits

• Ready-to-transduce lentivirus in three to four weeks
• Ultra-high titer virus made for in vivo and stem cell applications
• Highly concentrated viral preparations
• Precise, active virus titer measurements by qPCR included
• Three different titer levels and flexible volumes available

Customer offer

Target gene information

We provide

The lentiviral packaging experiment report provides 1*108 TU/ml virus (1 ml), control virus (200 μl), and the like.

Service specification

Services		Quantity	results	Cycle
Vector construction	ShRNA vector of the target gene	1	Report	1 to 2 weeks
Vector construction	Target gene shRNA vector Subscription	5	Report(4 object +1 negative contrast)	2 to 3 weeks
siRNA lentiviral screening	Buy cell recovery and training	1	Cell state Image	2 working days
	Slow virus infection experiments	1	The infection efficiency images and analysis	3 working days
	shRNA design	3	Target information	1 working days
	shRNA vector construction	4	Vector Construction Report	2 to 3 weeks
	Lentiviral packaging (10^8TU/ml)	5	The virus liquid and the titer test report	2 to 3 weeks
	The slower virus infection ( interference filter)	4	Picture	1 to 2 weeks
	qRT-PCR primer design and verification	2	Primer sequences ( containing an internal reference )	1 weeks
	qRT-PCR detect	3*5	Experimental data and analysis	1 to 2 weeks
	Western Blot detect	5	Experimental data and analysis ( primary antibody )	1 to 2 weeks
	Total price	/	/	7 to 10 weeks
Establish stable Filter cell lines	The effective siRNA lentiviral repeated infection ( positive + negative )	2	Picture	1 to 2 weeks
	Stable cell strain was amplified ( positive + negative )	2	Cell picture	3 to 4 weeks
	qRT-PCR detect	3*2	Experimental data and analysis	1 to 2 weeks
	Western Blot detect	3*2	To provide an anti- experimental data analysis (customer )	1 to 2 weeks
	Infected cells (25T flasks , positive ( two kinds ) + negative , each bottle )		Cells and photo	1 to 2 weeks
	Total	/	/	6 to 9 weeks

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200 µL Lentivirus Stock Custom Lentivirus Production Molecular Genetic Analysis Images

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200 µL Lentivirus Stock Custom Lentivirus Production Molecular Genetic Analysis

200 µL Lentivirus Stock Custom Lentivirus Production Molecular Genetic Analysis

Custom Lentivirus Production Molecular Genetic Analysis

Lentivirus Stock Molecular Genetic Analysis

200 µL Molecular Genetic Analysis

200 µL Lentivirus Stock Custom Lentivirus Production Molecular Genetic Analysis Images